Lab #______ Name______________________

Per_______ Date___________

Sexual Reproduction in Flowering Plants

Introduction

The angiosperms are seed-bearing plants that produce flowers. The seeds, which contain the plant embryo, are produced in the flower. All the parts of a flower are actually modified leaves that are specialized for their roles in the reproductive process. Flower parts are arranged in circles called whorls. They are attached at the enlarged base of the flower, the receptacle.

Flower structures can be divided into two groups: the essential organs and the accessory organs. The essential organs are the reproductive structures, which include the stamens (male) and the pistils (female). The accessory organs are the sepals and petals, which surround and protect the essential organs.

The stamen is the male reproductive organ and consists of two parts: the anther and the filament. The anther

is the enlarged structure at the top of the stamen. Inside the anther are pollen sacs. Special cells within the pollen sacs undergo meiosis to form pollen grains. Each pollen grain contains two sperm nuclei. When the pollen grains mature, the pollen sacs split open to release the dust-like pollen. The filament is a thin stalk that supports the anther.

The pistil is the female reproductive organ and consists of three parts: the stigma, style, and ovary. The stigma is an enlarged portion at the top of the pistil that becomes moist and sticky when mature. The style is the middle portion of the pistil. It can be long and slender, short, or even absent, depending upon the species. The ovary is the enlarged structure at the bottom of the pistil. The ovary contains one or more hollow compartments called locules. Each locule contains one or more ovules. Special cells within the ovule undergo meiosis to form ova (eggs) containing egg nuclei.

Pollination occurs when pollen grains land on the sticky surface of the stigma and are trapped there. The pollen grain germinates and a pollen tube emerges from the grain. It releases special enzymes that digest a cell the wall on the surface of the stigma. The pollen tube grows down through the style to the ovary and enters the ovule, making a continuous passageway for the two sperm nuclei to enter the ovum. Fertilization occurs when the sperm nuclei join the egg nuclei.

The fertilized egg becomes an embryo. The wall of the ovule thickens and forms a seed, thus enclosing and protecting the embryo. The ovary wall also thickens and develops into a fruit. In some plants such as apples, the ovary walls become fleshy and contain stored sugars and starches. In other plants such as walnuts, the ovary walls become dry and hard.

Purpose

1. To study the structure of a typical flower.
2. To study the male and female reproductive organs needed for sexual reproduction in flowering plants.

Materials

fresh flower(s) dissecting needle

hand lens microscope scalpel or razor blade

plain paper forceps

clear tape metric ruler

Procedure

1. Cut out the Chart on the last page of this handout and tape it into your lab

databook under the heading "Observations".

2. Obtain a single flower and observe its parts carefully. Flower parts are

arranged in a circular pattern. Each circle is called a whorl. The whorls are

attached at the enlarged receptacle located at the base of the flower.

Please read this overview before you begin your flower dissection:

As you examine your flower, you will be carefully removing parts beginning

with the outer whorl and working your way in towards the pistil. You will

arrange each whorl in a circle on the plain paper, beginning with the sepals as

the largest outermost circle. As you proceed with your dissection, you will

carefully tape each whorl of flower parts into position and label them (please

use pencil!). As each whorl is observed and removed, you will complete the

appropriate information in the Observations column of the chart. Use the

information in the handout to complete the Function column of the chart.

3. The sepals form the outermost whorl of the flower. The sepals are leaf-like

structures that are usually green in color. Sometimes, the sepals are the

same color as the petals, or appear to be another set of petals of a different

color. The function of the sepals is to protect the inner part of the flower

before it blossoms. Gently remove the sepals, tape them into position onto

the paper, and label them. On the chart, record the following observations:

a) How many sepals does your flower have?

b) Describe the appearance of the sepals (color, markings, etc.).

4. The petals are found directly under the sepals. The color and odor of the

petals help to attract birds and insects to the flower for pollination. Gently

remove the petals, tape them into position onto the paper, and label them.

On the chart, record the following observations:

a) How many petals does your flower have?

b) Describe the appearance of the petals (color, markings, etc.).

5. The stalk-like structures inside the petals are the stamens, the male

reproductive organs. Depending on the species, the stamens may be

attached to the receptacle, to the petals, or to the pistil. The enlarged portion

at the top of the stamen is the anther. Inside the anther are pollen sacs,

which produce pollen grains. When the pollen grains mature, the pollen

sacs split open, releasing the dust like pollen grains. The filament is the thin

structure that supports the anther. Gently remove the stamens, tape them

into position onto the paper, and label them. On the chart, record the

following observations:

a) How many stamens does your flower have?

b) To which structure(s) were the filaments attached?

c) Have the pollen sacs opened? How can you tell?

d) If pollen grains are visible, describe their appearance.

6. The central structure of the flower is the female reproductive organ, the pistil.

The top of the pistil is the stigma. When mature the stigma is enlarged, and

its surface is moist and sticky. The style is the middle portion of the pistil. It

supports the sigma. Some flowers lack a style. The ovary is the enlarged

structure at the bottom of the pistil. The ovary contains one or more hollow

compartments known as locules. The locules contain ovules, which in turn,

contain the egg nuclei. Carefully remove the pistil by cutting it from the

stem just under the ovary. Using pencil, make a life-sized sketch of the

entire pistil (just the outline) in the center of the plain paper and label it. Cut

the style just at the top of the ovary, tape it next to your sketch, and label the

stigma and style. Using the ruler, measure the length of the style in

millimeters. Cut a thin cross-wise section of the ovary and tape it under

the stigma and style. Label the ovary wall, locules and ovules. Using the

dissecting needle, carefully pick the ovules out of one of the locules. Try

to estimate how many ovules are contained in one locule. On the chart,

record the following observations:

a) What color is the pistil?

b) Describe the appearance of the stigma. Is the stigma mature? How can you tell?

c) How long is the style (in mm)?

d) Describe the appearance of the ovary.

e) How many locules does the ovary contain?

f) Approximately how many ovules are contained in one locule?

7. Copy the statement "Teacher approval of flower dissection:______"

into your lab databook and ask your teacher to check your work. Your

teacher will initial your databook and collect your flower paper if:

· All flower parts are correctly taped in place.

· All flower parts are labeled correctly (in pencil).

· The pistil is drawn on the paper (in pencil).

· Your name(s) and class period are written on the paper.

· The Observations column of your chart is completed.

Discussion - Please write the answers to the following questions in your lab databook using complete sentences.

1. Which does your flower produce in greater numbers: ovules or pollen grains? Explain why this would be important in terms of reproductive success.

2. What are some adaptations of flower petals to help attract pollinators?

3. How is the stigma of your flower adapted to capture and hold pollen?

4. Sometimes, pollen from a different species lands on the stigma of a flower. Based on your knowledge of cell communication, suggest a mechanism that would ensure that only the correct species of pollen germinates on the stigma of a particular type of flower.

5. a) Describe where pollination and fertilization occur.

b) Explain the differences between pollination and fertilization.

6. a) In which part of the male reproductive organ are the pollen grains

made?

b) In which part of the female reproductive organ are the egg cells made? c) By which nuclear process are these gametes formed?

7. How do the sperm nuclei in a pollen grain reach the egg nucleus in an

ovule?

8. a) Which part of the flower becomes the seed?

b) Which part becomes the fruit?

c) Which part of the fruit contains the embryo?

Conclusion

Summarize the information presented in this lab by creating a brief outline and writing it in your lab databook. Your outline should include information about types of flower parts, male and female structures, pollination, fertilization, and development of seed and fruit. Use the Introduction as your guide!

Please cut out this chart and tape it into your lab databook under the heading "Observations"

Structure	Function	Observations
Receptacle
Sepals		a) ________ b)
Petals		a) ________ b)
Stamens		a) ________
Filament		b) _________________
Anther		c)
Pollen grains		d)
Pistil		a) _________________
Stigma		b)
Style		c) ____________
Ovary		d)
Locule		e) ________
Ovule		f) ________